Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus
Identifieur interne : 002F47 ( Main/Exploration ); précédent : 002F46; suivant : 002F48Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus
Auteurs : LILI XU [République populaire de Chine] ; YANFANG ZHANG [République populaire de Chine] ; YUN LIU [République populaire de Chine] ; ZHIWEI CHEN [Hong Kong] ; HONGKUI DENG [République populaire de Chine] ; ZHONGBIN MA [République populaire de Chine] ; HUALIN WANG [République populaire de Chine] ; ZHIHONG HU [République populaire de Chine] ; FEI DENG [République populaire de Chine]Source :
- Journal of general virology [ 0022-1317 ] ; 2009.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4 % to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks.
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Zhang" sort="Yanfang Zhang" uniqKey="Yanfang Zhang" last="Yanfang Zhang">YANFANG ZHANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan 430071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yun Liu" sort="Yun Liu" uniqKey="Yun Liu" last="Yun Liu">YUN LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan 430071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhiwei Chen" sort="Zhiwei Chen" uniqKey="Zhiwei Chen" last="Zhiwei Chen">ZHIWEI CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>AIDS Institute, The University of Hong Kong Li Ka Shing Faculty of Medicine</s1><s3>HKG</s3><sZ>4 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>AIDS Institute, The University of Hong Kong Li Ka Shing Faculty of Medicine</wicri:noRegion></affiliation></author><author><name sortKey="Hongkui Deng" sort="Hongkui Deng" uniqKey="Hongkui Deng" last="Hongkui Deng">HONGKUI DENG</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Cell Biology and Genetics, College of Life Sciences, Peking University</s1><s2>Beijing 100871</s2><s3>CHN</s3><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Zhongbin Ma" sort="Zhongbin Ma" uniqKey="Zhongbin Ma" last="Zhongbin Ma">ZHONGBIN MA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan 430071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Hualin Wang" sort="Hualin Wang" uniqKey="Hualin Wang" last="Hualin Wang">HUALIN WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan 430071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan 430071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Fei Deng" sort="Fei Deng" uniqKey="Fei Deng" last="Fei Deng">FEI DENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Wuhan 430071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological receptor</term><term>Coronavirus</term><term>Microbiology</term><term>Protein</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Récepteur biologique</term><term>Protéine</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term><term>Angiotensin converting enzyme 2</term><term>Nyctereutes procyonoides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4 % to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country><region><li>Hubei</li><li>Pékin</li></region><settlement><li>Pékin</li><li>Wuhan</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Lili Xu" sort="Lili Xu" uniqKey="Lili Xu" last="Lili Xu">LILI XU</name></region><name sortKey="Fei Deng" sort="Fei Deng" uniqKey="Fei Deng" last="Fei Deng">FEI DENG</name><name sortKey="Hongkui Deng" sort="Hongkui Deng" uniqKey="Hongkui Deng" last="Hongkui Deng">HONGKUI DENG</name><name sortKey="Hualin Wang" sort="Hualin Wang" uniqKey="Hualin Wang" last="Hualin Wang">HUALIN WANG</name><name sortKey="Yanfang Zhang" sort="Yanfang Zhang" uniqKey="Yanfang Zhang" last="Yanfang Zhang">YANFANG ZHANG</name><name sortKey="Yun Liu" sort="Yun Liu" uniqKey="Yun Liu" last="Yun Liu">YUN LIU</name><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name><name sortKey="Zhongbin Ma" sort="Zhongbin Ma" uniqKey="Zhongbin Ma" last="Zhongbin Ma">ZHONGBIN MA</name></country><country name="Hong Kong"><noRegion><name sortKey="Zhiwei Chen" sort="Zhiwei Chen" uniqKey="Zhiwei Chen" last="Zhiwei Chen">ZHIWEI CHEN</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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